In Vitro Digestibility of Soybean Meal, Feather Meal and Palm Kernel Cake with Mannanase BS4 and Bromelain Cocktail Enzymes Addition
Tuti Haryati a), Arnold P Sinurat b), Wisri Puastuti c), Ferdy Saputra d), Diana Andianita Kusumaningrum e) and Nila Miraya f)

Research Center for Animal Husbandry, Research Organization for Agriculture and Food, National Research and Innovation Agency, Bogor 16915, Indonesia

Abstract

The addition of mannanase BS4 or protease enzymes can improve dry matter and protein digestibility on feedstuffs such as soybean meal (SBM) and palm kernel cake (PKC). The purposes of this research were to evaluate the effect of mannanase BS4 and bromelain additions on in vitro dry matter and protein digestibilities of SBM, feather meal (FM), and PKC and to determine the optimum composition of cocktail enzymes addition. In vitro digestibility values with each enzyme addition were compared with the one without any enzyme addition. Synergistic activities of cocktail enzymes were evaluated with the mixture of mannanase BS4 and bromelain addition (100:0- 0.75:0.25- 0.50:0.50- 0.25:0.75- 0:100 dosage) with 6 replicates. All in vitro dry matter digestibility (DMD) values of feedstuffs with each enzyme addition were higher than that of the control. Mannanase BS4 increased DMD and in vitro protein digestibility (IVPD) on SBM, FM and PKC respectively for 0.26-1.06x and 0.13-0.98x, while bromelain addition increased for 0.11-1.31x and 0.12-1.17x. Synergistic activity was only observed at 0,25:0,75D mannanase BS4 and bromelain in PKC, proved by the increasing DMD for 1.49x. However, synergistic activities at CPD were not observed. In conclusion, the addition of enzymes could improve DMD and IVPD on feedstuffs. The addition of each enzyme in SBM and FM showed better results than the addition of cocktail enzymes. Even though CPD synergistic activities were not observed on PKC, the DMD increasement might be enhance feed efficiency for in vivo trials.

Keywords: in vitro, digestibility, soybean meal, feather meal, palm kernel cake, mannanase BS4 and bromelain

Topic: Animal Nutrition and Feed Technology