Effect of Slow Freezing or Rapid Freezing With Different Concentrations of Dimethyl Sulfoxide on Sperm Quality of Indonesian Local Chicken Post Thawing
Khaeruddin, Gatot Ciptadi, Muhammad Yusuf, Sri Wahjuningsih

Faculty of Animal Science, University of Brawijaya

Abstract

This study aimed to determine the effect of rapid and slow freezing with various concentrations of dimethyl sulfoxide (DMSO) on the sperm quality of Indonesian local chickens after thawing. This study used a completely randomized design with 5 levels of treatment, namely slow freezing DMSO 7%, slow freezing DMSO 14%, rapid freezing DMSO 7%, rapid freezing DMSO 14% and rapid freezing DMSO 21%. The data were analyzed for variance (ANOVA), if a significant effect was found, it was continued with Duncan^s Multiple Distance Test. Before freezing, fresh semen from rooster was evaluated macroscopically and microscopically. For the slow freezing treatment, the packaged semen was placed in equilibration then proceeded to the pre-freezing and freezing stages. Meanwhile, the fast freezing treatment directly enters the pre-freezing stage and then freezes without going through an equilibration process. Motility, viability and abnormalities were evaluated after thawing. The results indicated that rapid and slow freezing did not affect sperm motility and abnormalities in Bangkok sperm after thawing, but did affect sperm viability, where rapid freezing with 14% DMSO concentration was able to improve sperm viability. So that the rapid freezing technique has the potential to replace the slow freezing technique in Indonesian local chicken semen cryopreservation.

Keywords: Slow freezing- Rapid freezing- DMSO- Chicken sperm

Topic: Animal Production