Elimination of Viral Systemic Disease in Shallot (Allium ascolonicum L) var. Bima Brebes through Unconventional Approach Asih K. Karjadi (1), Nazly Aswani (1), Neni Gunaeni (1)
1Research Center for Horticultural and Estate Crops, Research Organization for Agriculture and Food, National Research and Innovation Agency, Cibinong Science Center, Jl. Raya Jakarta-Bogor, Cibinong, Bogor 16915, West Java, Indonesia
*Corresponding email: asihkk [at]yahoo.com- asih005[at]brin.go.id
Abstract
Shallot (Allium ascolonicum L), belonging to the genus Allium are propagated vegetatively through layered bulbs. This research aimed to improve the quality of shallots by eliminating OYDV, SLDV viruses through meristematic tissue combined with antiviral Ribavirin. The research was carried out in the IVEGRI Tissue Culture Laboratory from April to August 2018. Research materials were infected shallot bulbs of Bima Brebes variety which were previously confirmed by DAS ELISA serology test. Meristematic tissues were cultured on MS + vitamin, MS + supplement media (sucrose 30 gL-1 + Myo inositol 100 mgL-1 + CaP 2 mgL-1 + NAA 0.15 mgL-1 + Kinetin 2 mgL-1 + GA3 0.1 mgL-1 + Gelgro 2 mgL-1, pH 5.7). The treatments included Ribavirin concentrations i.e., 0.5,10,15, 20, 30 mgL-1 and explant size including meristematic tissue (0.5 mm) and shoot tip (2.5 mm). A Completely Randomized Design with ten replications was used for this research. The results obtained on visual observation showed that the addition of antiviral Ribavirin and explant size affected the percentage of growth and development of plantlets. Statistical analysis showed that there were no interactions between the concentration of Ribavirin and explant size. DAS Elisa serology test showed that the higher the concentration of Ribavirin the lower the percentage of virus infection.
