The Evaluation of Antagonistic Fungi in Inhibiting Fusarium verticillioides Growth AM Yasir, T Kuswinanti, A Nasruddin
Department of plant pests and diseases, Faculty of Agriculture, Hasanuddin University
Makassar, Sulawesi Selatan 90245, Indonesia
Abstract
Fusarium verticillioides the cause of stem rot in maize which causes a decrease in yield and quality of maize. The rapid spread of pathogens has resulted in the need for Fusarium so proper and safe. The purpose of this study was to test the effectiveness of fungal isolates which are antagonistic in inhibiting the growth of F. verticillioides Purify isolates of antagonist fungi available at the Laboratory of Plant Pests and Diseases, Hasanuddin University, namely Penicillium sp, Aspergillus niger, Aspergillus flavus, and Trichoderma harzianum. Place theF. verticillioides isolate and the antagonist fungus according to the dual culture method. Measure the growth radius of each fungus until the F. verticillioides as control fills the petri dish. Calculating the inhibition of antagonistic fungi to see the effectiveness of fungi that can potentially be antagonistic fungi. Seeing the interaction mechanism that occurs by taking the hyphae of the meeting between the two fungi and observing using a microscope. The research was conducted at the Laboratory of Plant Pests and Diseases, Hasanuddin University. Based on the servitude test between antagonistic fungi, it is known that the fungus that has the best potential to suppress the growth of F. verticillioides is the fungus Trichoderma harzianum with a high category of 60.78% with competition and parasitism mechanisms and the fungus Aspergillus flavus 52.94% high category with a mechanism antibiotics. Meanwhile, the fungi included in the medium category were Aspergillus niger 49.41% with the antibiosis mechanism and the fungus Penicillium 47.06% and no interaction was found due to the growth of the dominant pathogen occupying the space.
