Indole-3-acetic acid associated with plant-growth promoting microorganisms in response to Arabidopsis seed growth and germination Muhammad Yusril Hardiansyah (a,d*), Vietgar Membalik (b,c), Andi Khusnul Fatima Bahar (c), Novaty Eny Dungga (d)
a) Global Agriculture Technology and Genomic Science, International College, National Taiwan University, Taipei 10617, Republic of China, Taiwan
*yusrilhardiansyah1[at]gmail.com
b) Wageningen University & Research, Droevendaalsesteeg 4, 6708 PB Wageningen, The Netherlands
c) Plant Protection Study Program, Faculty of Agriculture, Universitas Hasanuddin, Makassar 90245, Indonesia
d) Department of Agronomy, Agrotechnology Study Program, Faculty of Agriculture, Universitas Hasanuddin, Makassar 90245, Indonesia
Abstract
Plant growth-promoting microorganism as a bacterial group such as PGPR is one of biological pathway which is widely associated with Indole-3-acetic acid (IAA). As the primary member of the auxins, IAA plays an essential role in the response of plant activities, including seed growth and development specifically. Extensive studies have been performed on IAA after its discovery as a plant hormone. It has been found out that different bacteria, fungi and algae are capable of producing physiologically active amounts of IAA and important for plant growth and development. The objective for this research is to decipher whether IAA can affect in seed growth and germination supposed to optimum growth of hypocotyl. By using plant growth-promoting microorganism which was colonized from the bamboo rhizosphere and as IAA^s roles, this group of microorganism was also coated into the seed of arabidopsis before germinate in the MS medium. The parameter observations include Seed germination rates and periods, the average number of seed germination, abnormal seed, hypocotyl growth with three biological replicates. The form of Tukey^s test (one-way ANOVA) with SD was used as statistical analysis. The results shown IAA was successfully increased seed germination rates during 24, 48, 72, 96, 120, 144 hours, by longer period, also successfully increased the germination rate until 5 days. Furthermore IAA also reduces abnormal seeds (5%), increased the number of seed germination, and enhanced hypocotyl growth in average. To sum up this study, IAA successfully involved in seed germination and enhance seed growth after coated. Consequently, IAA plays an important role in response to seed growth and germination in Arabidopsis.
