The effect of ethyl methansulfonate concentration and soaking time of anthurium plowmanii croat seeds in vitro
Dita dindasari(1), Rinaldi sjahril(2), Ifayanti ridwan(3)

(1) Master Student, Agrotechnology Program Study, Hasanuddin University, Makassar, 90245, Indonesia
(2) Laboratory of Plant Bio-science and Reproduction Biotechnology, Faculty of Agriculture, Hasanuddin University, Makassar, 90245, Indonesia
(3) Laboratory of Plant Ecophysiology and Nutrition, Hasanuddin University, Makassar, 90245, Indonesia

Abstract

Anthurium plowmanii Croat is one of horticultural commodities that has high economic value and varieties, hence the diversity that need to be developed. This research aimed to determine the effect of EMS concentration and soaking time on the growth of Anthurium plowmanii Croat in vitro. Research activities were carried out at the Laboratory of Biosciences and Plant Reproductive Biotechnology, Teaching Industry, Universitas Hasanuddin from July to December 2021. The study used a two-factor factorial completely randomized design (CRD). Research activity was carried out by soaking the seeds of the Anthurium plowmanii Croat at several concentrations of EMS (0%, 0.025%, 0.05%, 0.1%, and 0.4%) for some immersion times (30 minutes, 60 minutes, and 90 minutes) resulted in a total of 15 treatment combinations. The results show that the concentrations of EMS and soaking time had a significant on the parameter leaves chlorophyll content. EMS caused a decrease germination on the number of leaves, number of roots and plant height in vitro.

Keywords: Anthurium plowmanii Croat, ethyl methanesulfonate, in vitro

Topic: Plant breeding and biotechnology