In Vitro Multiplication of ^Kepok^ Banana (Musa acuminata x Musa balbisiana) using different concentration of BAP and NAA in MS media
Ifayanti Ridwan1, Warni Safitri2, Amir Yassi1, Muh. Farid BDR1, Yunus Musa1, Mansyur3, and Idarni Tenripada3

1Department of Agronomy, Faculty of Agriculture, Hasanuddin University. Jl. Perintis Kemerdekaan KM 10 Makassar 90245 Indonesia.
2Agrotechnology Study Program, Faculty of Agriculture Hasanuddin University. Jl. Perintis Kemerdekaan KM 10 Makassar 90245 Indonesia.
3Tissue Culture Laboratory, Horticultural Plant Seed Center, Bonto-bonto District, Gowa Regency.

Corresponding author^s e-mail address: ifayanti[at]unhas.ac.id

Abstract

This study aims to determine the effect of different composition of BAP and NAA concentration added to Murashige and Skoog (MS) basic media on the in vitro multiplication of Indonesian ^Kepok^ banana variety. The research was conducted at the Tissue Culture Laboratory of the Bonto-Bonto Horticultural Plant Seed Center. A completely randomized design (CRD) was employed with two factors tested. Four concentrations of each BAP and NAA used were 0 ppm, 4.5 ppm, 5 ppm, and 5.5 ppm for BAP, and 0 ppm, 2 ppm, 2.5 ppm, and 3 ppm for NAA resulted in 15 composition of BAP:NAA in addition to control (without the hormones). The results show no interaction between the BAP and NAA treatments, BAP concentration of 5 ppm gave the best result on parameter time of shoots appearance (7.50 DAP). NAA concentration of 2.5 ppm gave the best results on root emergence time (8.61 DAP), plantlet growth time (11.72 DAP), number of roots (4.81 fruit) and root length (5.93 cm).

Keywords: In vitro, BAP, NAA, banana, kepok.

Topic: Crop production system