|
Characterization of Amylolytic Bacteria Isolated from Macroalgae at Sepanjang Beach, Yogyakarta 1) Biology Study Program, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta. Jl. Pemuda No. 10 Rawamangun, East Jakarta, Indonesia 13220 Abstract Enzymes are important components in microorganisms. One of the enzymes commonly produced is amylase. Amylase enzymes have various uses, such as basic material in the food and drug industry, the textile industry and other industries. Amylolytic bacteria has the ability to hydrolyze starch or into simpler compounds. This study aims to obtain amylolytic bacteria associated with macroalgae in Sepanjang Beach, Gunungkidul, Yogyakarta. The 3 macroalgae samples studied were Chaetomorpha linum, Eucheuma spinosum, and Enteromorpha sp.. There were 10 colony bacteria isolated from Chaetomorpha linum, 8 colony from Eucheuma spinosum, and 8 colony from Enteromorpha sp.. Isolation of bacteria using pour plate method on Marine Agar medium with incubation for 24 hours. Selection of amylolytic bacteria was carried out on 2% Starch Agar medium. A positive result was indicated by the presence of a clear zone around the colony after added iodine reagent. The amylase production ability of each bacterial isolate was tested by measuring the Amylolytic Index. Characterization of bacterial isolates was carried out based on observations of colony morphology (shape, size, color, edge, and elevation), as well as cell morphology which included cell shape and Gram type. Amylolytic index data were analyzed quantitatively using Duncan^s multiple-region union, and data on the characteristics of bacterial isolates were analyzed qualitatively. Keywords: Amylolytic bacteria, characterization, macroalgae Topic: Biology |
| SMIC 2022 Conference | Conference Management System |