Screening and Isolation of Indigenous Cellulolytic and Proteolytic Yeasts From Fermented Dried Cocoa Beans
Dalia Sukmawati1,2, Shabrina Nida Al Husna3, Desty Saszieta1, Almira Marvella Priskaningrum1, Axel Mareta Mutiani1, Ariza Budi Tunjung Sari4 and Hesham Ali El-Enshasy5

1Department of Biology, Faculty of Mathematics and Natural Sciences, Hasyim Ashari Building, 9th floor, Universitas Negeri Jakarta, Jakarta, Indonesia.Dalia-Sukmawati[at]unj.ac.id
2Universitas Negeri Jakarta Culture Collection (UNJCC), Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Rawamangun, Indonesia- Dalia-Sukmawati[at]unj.ac.id
3Department of Microbiology, School of Life Science and Technology, Institut Teknologi Bandung, Bandung, Indonesia.
4Coffee and Cocoa Research Center Indonesia, Jl. PB Sudirman No.90, Wetan Ktr., Jemberlor, Kec. Patrang, Kabupaten Jember, Jawa Timur 68118, ariza.bts[at]gmail.com
5Institute of Bioproduct Development, Universiti Teknologi Malaysia (UTM), Skudai, Johor Bahru, Malaysia- henshasy[at]ibd.utm.my

Abstract

Fermentation is a biochemical process that requires various microorganisms. The activity of microorganisms in the cocoa bean fermentation process is essential to successful enzymatic molecule breakdown. Yeast is a functional microorganism in cocoa bean fermentation and acts as a glucose and ethanol producer. Yeast has the ability to produce cellulase and protease enzymes, which are both frequently used in industry. This study aimed to screen and isolate yeast that can degrade cellulose and proteases from the fermentation of Sulawesi 1 and TSH 858 cocoa beans. The study was carried out using the descriptive method. Data were obtained by isolating up to 20 g of yeast from each fermentation. The results showed 231 yeast isolates, including 136 from Sulawesi 1 and 95 from TSH 858. The cellulolytic screening revealed 29 isolates from Sulawesi 1 and 27 isolates from TSH 858, whereas the proteolytic screening discovered three isolates and no isolate from the TSH 858 that produced proteolytic enzymes.

Keywords: screening, yeast isolation, cellulolytic. proteolytic, cocoa bean fermentation

Topic: Biology