Screening of Factors Influencing Riboflavin Production by Recombinant Ashbya Gossypii Using Plackett-Burman Fractional Factorial Design Experiment
Nivashini Neela Mekan (a),(b), Tatsuya Kato (c), Enoch Y Park (c), Daniel Joe Dailin (a),(b), Hesham Ali El-Enshasy (a),(b),(d)*

a) Institute of Bioproduct Development, Universiti Teknologi Malaysia, Skudai, Johor, Malaysia.
b) School of Chemical and Energy Engineering, Faculty of Engineering, Universiti Teknologi Malaysia, Skudai, Johor, Malaysia.
c) Laboratory of Biotechnology, Major in Bioscience, Graduate School of Science and Technology, Shizuoka University, Suruga-ku, Shizuoka, Japan.
d) City for Scientific Research and Technology Applications, New Burg Al Arab, Alexandria Egypt.
*henshasy[at]ibd.utm.my

Abstract

Riboflavin is an intrinsic component for significant nutritional and growth factor of living organisms. It was fermentatively synthesized from different microorganisms such as Ashbya gossypii, Eremothecium ashbyii, Candida famata and Bacillus subtilis. This water-soluble vitamin is a part of commercial interest since, the earliest decade when human health was in threat. Despite to its multiple utilities in all market sectors, the global riboflavin market is seeming to increase its demand, annually. To that, findings pertaining various environmental and nutritional factors contributing riboflavin yield were improvised with a cost-effective statistical experimental design. Previously, the individual effect of component on riboflavin production was evaluated via One-Factor-at-a-Time (OFAT) method. Using recombinant Ashbya gossypii strain, the combined effects of six variables (sunflower oil, corn steep liquor, yeast extract, glycine, potassium phosphate, and Tween 60) were assessed in Plackett-Burman Design. A total of five statistically significant (P<0.05) factors - sunflower oil, corn steep liquor, yeast extract, glycine and tween 60 were identified. The statistical analysis proposed that the linear mathematical model used for prediction is significant with the coefficient of determination R2(0.8774). Comparatively to the basal medium, 55% of higher metabolite was achieved based on the model of the optimized medium.

Keywords: Ashbya gossypii, Plackett-Burman Design, riboflavin, vitamin.

Topic: Biology