THE OVERVIEW OF MICROBIOLOGICAL QUALITY OF THE UPSTREAM OF CIKAPUNDUNG RIVER BASED ON TOTAL COLIFORM AND Escherichia coli
Eka Noneng Nawangsih, Ania Kurniawati, dr., M.Kes, Dinyar Supiadi W, Muhammad Azka Fadhlan Qinthara

Faculty of medicine, Universitas Jenderal Achmad Yani, Cimahi

Abstract

Cikapundung River is one of many rivers which its existence used by Bandung citizens for many function. One of the function is main water source for Dago Pakar Water Treatment Plant which is located in Bantar Awi. The social activity as a cow farmer can contaminate the quality of Cikapundung River because of the disposal of the waste that didn^t go through the process. The purpose of this research was to determine the amount of Total Coliform Bacteria and Escherichia coli contained in the upstream of Cikapundung River and to adapt with the regulation of the minister of the health of the Republic of Indonesia Number 32 of 2017. This research is a descriptive study conducted at Microbiology Laboratory, Faculty of Medicine in Jenderal Achmad Yani University. The sample was collected from three spots: upstream, midstream and downstream on three times which are morning, daylight, and afternoon. The method used is Most Probable Number with three steps including Presumptive test using Lactose Broth, Confirmed Test using BGLB (Brillian Green Lactose Broth) and Completed Test using EMBA (Eosin-methylene blue agar). The presumptive test showed all samples are positive except for the first spot which is from the upstream as main water source. The confirmative test showed on second and third spot taken on morning, daylight and afternoon contain very high amount of coliform which is 1101 CFU/100 ml. On completed test, E. coli, Enterobacter and Klebsiella colonies were found. It can be concluded that microbiological quality of Cikapundung River is not yet fullfiled the standard.

Keywords: Cikapundung River, coliform, most probable number

Topic: Epidemiology and Public Health