Detection of White Spot Syndrome Virus (WSSV) and Infectious Hypodermal and Haematopoietic Necrosis Virus (IHHNV) in Green mussel (Perna viridis) Linnaeus 1758
1Sriwulan sriwulan, 1Amriana amriana, 1Arini Tri Jayanti, 1Hilal Anshary

1Laboratory of Fish Parasites and Diseases, Aquaculture Study Program, Department of Fisheries, Faculty of Marine Science and Fisheries, Hasanuddin University, Makassar, Indonesia. Corresponding author: Sriwulan sriwulan, sriwulancinga07[at]gmail.com

Abstract

White Spot Syndrome Virus (WSSV) and IHHNV (Infectious Hypodermal and Haematopoietic Necrosis Virus) are pathogens damaging shrimp aquaculture. Viral diseases can generate economic losses in shrimp farming activities. The aim of this study is to detect the occurrence of White Spot Syndrome Virus (WSSV) and Infectious Hypodermal and Haematopoietic Necrosis Virus (IHHNV) in green mussel (Perna viridis) Linnaeus 1758. This research was conducted in March-September 2016. A total of 20 samples of green mussels were taken from Takalar and Pangkep regency. The organs observed were gills, gonads, muscles, and hepatopancreas. The stages of this study included sample preparation, DNA extraction, DNA amplification, and electrophoresis. Each sample was analysed by PCR technique using WSSV 436 F / R specific primers and IHHNV 309 F / R. The results of this study show that WSSV is positively detected on Pangkep and Takalar Regency, while IHHNV only detected in the Pangkep Regency. The prevalence of WSSV is 15% both in Takalar and Pangkep regency while IHHNV prevalence is 15% in Pangkep Regency. WSSV was determined on gill and hepatopancreas in Takalar Regency but only on gill in Pangkep while IHHNV was found in the gills in Pangkep waters. The existence of these diseases has indicated that green mussel can be accumulated the WSSV and IHHNV to transmit into the water environment.

Keywords: WSSV, IHHNV, Green mussel, Perna viridis, Gills, Hepatopancreas.

Topic: Aquatic Ecology and Conservation (International)