Formulation and Antibacterial Activity Test of Ethanol Extract of Angasana Leaves (Pterocarpus indicus Willd) Cream Against of Staphylococcus aureus
Nelly Suryani1, Narti Fitriana2, Vivi anggia1, Tina Yuliana1

1Pharmacy Departement, Faculty of Health Science, UIN Syarif Hidayatullah, Jakarta, Tangerang Selatan 15419, Indonesia

2Biology Departemen, Faculty of Science Technology UIN Syarif Hidayatullah, Jakarta, Tangerang Selatan 15419, Indonesia

Abstract

Angsana leaves (Pterocarpus indicus Willd) is one of the plants that contain chemical subtances such as alkaloid, flavonoid, saponin, tannin and triterpenoid that have antibacterial activity. This study aimed to formulate Cream of angsana leaves (pterocarpus indicus willd) ethanolic extract with vanishing cream base with tween 80 and span 80 as emulgator and to test the physical and antibacterial activity against the Staphylococcus aureus. The angsana leaves was extracted using ethanol 70 % by maseration method.Phytochemical compound were screened with standard method, and followed by antibacterial test with disk diffusion method. Ethanol extract of angasana leaves was made in 5 concentration series 5%, 10%, 20%, 30% and 40%. Ethanol extract of angasana leaves with the concentrations of 5% and 10 % have a moderate antibacterial activity and concentrations of 20%, 30% and 40% have a strong antibacterial activity against of staphylococcus aureus. The extract was formulated into cream preparations and evaluated for physical properties including organoleptic, homogeneity, pH value, dispersibility, viscosity and centrifugation test with the freeze-thaw cycling test method. The cream preparation test was carried out on day 0 and after the freeze-thaw cycling test. The antibacterial activity of the ethanol extract of angasana leaves cream against staphylococcus aureus on each preparation were as follows : F1 had a strong one of 11.15 mm- F2 and F3 had a moderate one of 10.15 and of 9.8 mm respectively. The antibacterial activity of the whole formula decreased after freeze-thaw cycling test.

Keywords: Ethanol Extract of Angasana Leaves- Cream- Staphylococcus aureus

Topic: Pharmaceutical Sciences