The potential effect of Torbangun (Coleus amboinicus Lour) leaves extract in decreasing of blood glucose levels and glutathione peroxidation activities in hyperglycemic rats model Meilla Dwi Andrestian1*, Rizal Damanik3, Faisal Anwar2, Nancy Dewi Yuliana4
1 Department of Nutrition, Poltekkes Kemenkes Banjarmasin, Indonesia
2 Departemen of Community Nutrition, IPB University, Indonesia
3 South Asian Food and Agricultural Science and Technology, IPB University, Indonesia
4 Departemen of Food Science, IPB University, Indonesia
Abstract
Glutathione peroxidase (GSH-Px) is one of the earliest and most powerful antioxidant enzymes in cell. GSH-Px can be used as an indicator of oxidative stress in people with diabetes mellitus. This endogenous antioxidant is able to increase its activity by giving antioxidants contained in the Torbangun leaves extract. This study was an experimental study with a completely randomized design using 25 Spraque Dawley rats. Rats were divided into four groups, namely NG (negative control, hyperglycemic rats, seven rats), N (normal, six rats), H-IM (hyperglycemic, control of metformin drugs 62.5 mg/kgBW, six rats), and H-IT (hyperglycemics, extracts of Torbangun 620 mg/kgBW, six rats). The H-IT group received a treatment of Torbangun leaves extract and the H-IM group received a treatment of metformin for 14 days. Blood glucose were taken on days 0, 4th, 7th, 11th, and 14th and blood serum GSH-Px levels were taken after rats in necropsy on day 15th. GSH-Px levels were measured from rat blood serum that had been decropped on day 15th. The results showed an increase in GSH-Px activity in the group of H-IT rats by 33.10% compared to the group of NG rats and a significant decrease in blood glucose levels (p = 0.005). Torbangun leaves extract plays a role in decreasing blood glucose levels, repairing β--cells pancreas, and increasing blood serum GSH-Px levels of hyperglycemic rats.
