Potential Effect Of Ethyl Acetate Extract Of Mimosa pudica L. Against Biofilm Formation Of Streptococcus mutans
Supnawadi(a), Bq. Mutmainnah(b)*

Akademi Kesehatan Gigi Karya Adi Husada Mataram

Abstract

The formation of S. mutans biofilms can survive in extreme environments and is more resistant to antibiotics. The ethyl acetate extract of M. pudica L. contains tannins and flavonoids which are known to inhibit the expression of the intercellular adhesion (ica) gene which is a regulator of S. mutans biofilm formation. The aim of the study was to compare the anti-biofilm ability of M. pudica at various concentrations of S. mutans biofilm. The methods used in this study include extraction of M. pudica L. by maceration method using ethyl acetate solvent, morphological and physiological analysis of S. mutans using the MicrobactTM. Potential inhibition of biofilm growth of S.mutans cells by Microtiter Plate Biofilm Assay method using ELISA reader and Total Plate Count. Surface morphological analysis of S. mutans biofilm was carried out by Scanning Electron Microscopy. The results showed that S. mutans formed the enzymes nitrate, glucose, mannitol, ONPG, urease, sucrose and catalase. Ethyl acetate extract of M. pudica L. could significantly inhibit biofilm formation with an effect of 78.7% with a Minimum Biofilm Inhibitory Concentration (MBIC) of 50 mg mL-1. The number of living bacterial cells in the biofilm of S. mutans cells was 81.2%. The benefits of the research are expected to be the ability of M. pudica L. extract as an antibiofilm against S.mutans and can be used as an alternative treatment for S. mutans infection.

Keywords: ethyl acetate extract, M. pudica L., biofilm formation, Streptococcus mutans

Topic: Medical Laboratory Technology