Ethyl Acetate And Ethanol Extract Of Leaf Abrus precatorius L. As Inhibitors Against Biofilm Formation Staphylococcus aureus Strains Methicillin Resistant Staphylococcus aureus (MRSA) 22372 Indonesia Local Isolate
Bq. Mutmainnah (a)*, Ni^matuzahroh (b), Afaf Baktir (c)

a) Akademi Kesehatan Gigi Karya Adi Husada Mataram, Indonesia*Bmmasadepan9[at]gmail.com
b), c) Faculty of Sains and Technology, Universitas Airlangga, Indonesia

Abstract

Isolate of Staphylococcus bacteria with code MRSA 22372 Indonesian Local Isolate (ILI) was derived from the urine of patients at RSUD Dr. Soetomo, Clinical Microbiology Installation Surabaya, Indonesia. The level of polarity of the solvent affects the inhibition of bacterial cell growth. The active ingredient of Abrus precatorius L. leaf extract has the potential to inhibit the growth of bacterial biofilms. This study aimed to compare the growth inhibitory activity of the bacterial biofilm MRSA 22372 ILI due to various treatments of ethyl acetate and ethanol extract of A. precatorius L. leaves from concentrations of 25 mgL-1 to 800 mgL-1. The results showed that the total flavonoid content of the ethyl acetate and ethanol extract of the leaves of A. precatorius L. were obtained respectively 241.67 mg CE/g and 205 mg CE/g. Ethyl acetate and ethanol extract of A. precatorius L. leaves inhibited MRSA 22372 ILI biofilm by 62.9% and 71.4%, respectively. Ethanol extract of A. precatorius L. leaf inhibited the growth of MRSA 22372 ILI biofilm higher than that of A. precatorius L. ethyl acetate leaf extract. Minimum Biofilm Inhibition Concentration (MBIC) value of MRSA 22372 ILI by ethyl acetate and ethanol extract of A. precatorius L. leaf 25 mg/L by 14.3%. Administration of ethyl acetate and ethanol extract of A. precatorius L. leaves at higher concentrations was required to achieve the eradication effect of MRSA 22372 ILI biofilm. The inhibitory effect of MRSA 22372 ILI biofilm growth by ethyl acetate and ethanol extract of A. precatorius L. leaves showed good inhibition in vitro, and further research is needed in clinical conditions in vivo.

Keywords: Ethyl acetate and ethanol extract, Abrus precatorius L., Inhibitor, biofilm, S. aureus, MRSA 22372 ILI

Topic: Medical Laboratory Technology