The effect of high fructose diet on body weight and interleukin-6 levels : study on Wistar rats
Josephine Thewakan(1*), Haerani Rasyid(1), Nurpudji A Taslim(1), Suryani Asad(1), Agussalim Bukhari(1), Aminuddin(1), Husni Cangara(2)

1. Department of Clinical Nutrition, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
* vivi.thewakan87[at]gmail.com
2. Department of Anatomical Pathology, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia

Abstract

Objective: This study aims to determine the effect of high fructose diet on the expression of interleukin-6 in Wistar rats.
Methods: This study was a laboratory experimental, randomized controlled design with a post test-only control group design on 30 adult male Wistar rats (Rattus novergicus) aged 8-10 weeks which were randomly selected and divided into control and intervention groups. Obesity status was achieved by giving AD II diet and fructose 25% ad libitum. Body weight measurement is carried out every week. At weeks 4, 8, and 12, the rats were sacrificed and blood samples were collected from the orbital sinus for interleukin-6 test.
Results: There was a significant increase in mean body weight of the 4-week HF group (p = 0.002), 8-week HF group (p = 0.034) and 12-week HF group (p = 0.004) rats. Body fat percentage of the 12-week HF group (p = 0.000) and obesity index / Lee index in the 12-week HF group (p = 0.043) rats were significant increase. There was also a significant positive correlation between body weight and body fat percentage to interleukin-6 level in Wistar rats fed with a high-fructose diet.
Conclusion: High fructose consumption can increase body weight, percentage of body fat which is positively correlated with interleukin 6 level.

Keywords: Fructose; Interleukin-6; Obesity

Topic: Dietetics and nutrition