SYNTHESIS OF GOLD NANOPARTICLES (AuNPs) WITH GLUTAMIC ACID SUPPORTING AGENT AS A COLORIMETRY SENSOR TO Salmonella Sp BACTERIA.
Azmalaeni Rifkah Ansyarif (a*), Abdul Karim (b), Paulina Taba (c), Wahidah Febriya Ramadhani (d)

a) Department of Chemistry, Hasanuddin University
Jalan Perintis Kemerdekaan KM 10, Kampus Unhas Tamalanrea, Makassar, South Sulawesi, Indonesi, 90245
*azmalaenirifkaha[at]gmail.com

Abstract

In this study, analyzed and synthesis the subject of gold nanoparticles (AuNPs) as a bacteria sensor for foodstuffs. The bacteria used in this study were Salmonella Sp bacteria. In this study, the synthesis of AuNPs used a glutamic acid as a blocking agent and ascorbic acid as reducing agent. Testing for AuNPs was carried out using UV-Vis with a wavelength of 450 - 600 nm. The test method used is a colorimetric method. This method is performed by analyzing the color change in the solution, namely the occurrence of a red color change with a wavelength of 530 nm. In testing AuNPs with Ascorbic Acid using UV-Vis was carried out with several mole ratios and the results were obtained at a wavelength of 525.40 nm and an absorption of 0.833 with a ratio of 1: 3. Meanwhile, AuNPs with Glutamic Acid were obtained at a concentration of 4 mM with a wavelength of 535 nm and an absorbance of 0.277. In testing of Glu-AuNPs with bacterial preparation of Salmonella sp in 10^8 CFU/mL - 10^6 CFU/mL concentration resulted in a shift towards the red absorption area.

Keywords: AuNPs, glu-AuNPs, Salmonella sp, ascorbic acid, UV-Vis

Topic: Science, education, and women