Isolation and the potential of bioactive protein from microsymbiont red algae Eucheuma cottonii as an antibacterial agents
Marinda (a*,#), Ahyar Ahmad (b, c*,#), Hasnah Natsir (b), Abdul Wahid Wahab (b), Nunuk Hariani Soekamto (b), Siti Fausiah (b), Yusriadi (a), Rafsanjany Ramadan (a), Harningsih Karim (d)

a)Master Program, Department of Chemistry, Faculty of Mathematics and Natural Sciences, Biochemistry Lab., Hasanuddin University, Perintis Kemerdekaan Street Km. 10 Tamalanrea, Makassar 90245, Indonesia *marinda.nr[at]gmail.com
b)Department of Chemistry, Mathematics and Natural Sciences Faculty, Hasanuddin University, Perintis Kemerdekaan Street Km. 10 Tamalanrea, Makassar 90245, Indonesia
c)Laboratory of Research Centre and Developing of Sciences, Faculty of Natural Sciences, Hasanuddin University, Perintis Kemerdekaan Street Km. 10 Tamalanrea, Makassar 90245, Indonesia *ahyarahmad[at]gmail.com
d)Department of Pharmacy, School of Pharmacy YAMASI, Mapala 2 Street Blok D5 No.10 Makassar 90222, Indonesia.

Abstract

Objective:The research of the potential of bioactive protein from microsymbiont red algae Eucheuma cottonii as an antibacterial has been carried out. This study aimed to know the antibacterial activity of bioactive protein from microsymbiont red algae.
Methods:The methods of research were fractionation, dialysis, and antibacterial activity test. The isolation of bioactive protein used ammonium sulphate fractionation method at the rate of 0-20%, 20-40%, 40-60%, and 60-80% percent saturation. Furthermore, the purification of bioactive protein was conducted by dialysis with a cellophane membrane. Antibacterial activity test using the agar diffusion method with testing bacterial against Staphylococcus aureus and Escherichia coli.
Results:The result showed that the most of inhibition zone towards S. aureus and E. coli showed that the fraction of 60-80 % saturation with a value of 13.20 mm and 10.66 mm, respectively.
Conclusion:The all of fraction have an inhibition zone towards the bacterial test.

Keywords: Antibacterial, Bioactive protein, E. cottonii, Microsymbiont

Topic: Medicinal plants and natural products