Predicting Anti Migratory Mechanism of Cucurbitacin E on Breast Cancer Cell : in Silico Analysis Inas Haidar1, Joko Setyono2, Muhamad Salman Fareza1, Sarmoko1*
1Department of Pharmacy, Faculty of Health Sciences, Jenderal Soedirman University
2Laboratory of Biochemistry, Faculty of Medicine, Jenderal Soedirman University
* Dr. Soeparno street, Karangwangkal, Purwokerto, Central Java, Indonesia, 53123. Email: sarmoko[at]unsoed.ac.id
Abstract
Breast cancer is the second most commonly diagnosed cancer worldwide. Invasion and metastasis are the main cause responsible for more than 90% of mortality. Several studies indicate that cucurbitacin E, an active compound of pumpkins, inhibits lung metastasis and blocks breast tumor cell migration and invasion. However, to date, no study has thoroughly investigated the functional mechanisms underlying the cucurbitacin E effects on the anti-migration of breast cancer cells. The goal of this study is to uncover the potential target and molecular mechanism of cucurbitacin E in inhibiting breast cancer migration. The bioinformatics approach by using STRING, STITCH, and PubMed was used to collect data containing possible target gene of cucurbitacin E. Genes affected by the cucurbitacin E retrievied from STITCH and PubChem were then compared with genes involved in breast cancer migration. A total of 31 possible therapeutic target genes of cucurbitacin E were found, then were further analyzed for gene ontology and KEGG pathway. The gene ontology data showed that these 31 protein targets participated in the biological processes of cell migration in response to chemokine through the sphingolipid signaling pathway. Taken together, we identified the potential mechanism of cucurbitacin E that may inhibit breast cancer cell migration.
Keywords: cucurbitacin E, breast cancer migration, sphingolipid signaling pathway
