SURVIVAL ANALYSIS OF INFECSIUS VECTOR DENV-2 THROUGH ARTIFICIAL INOCULATION Isna Hikmawati1, Hendro Wahjono, 2, Martini Martini3, Sjamsul Huda4
1Faculty of Health Sciences, Universitas Muhammadiyah Purwokerto, 2Faculty of Medical, University Diponegoro Semarang, 3Faculty of Public Health, University, Diponegoro Semarang, 4 MTs Negeri 2 Banyumas
Abstract
Introduction : Aedes aegypti and Aedes albopictus is the main vector of dengue fever, both of which have an important role in dengue transmission because they can simultaneously transmit the dengue virus vertically or horizontally. The survival vector causes the spread of the dengue virus to become endemic in an area. This study aims to determine the survival analysis, gonotropic cycle and metamorphosis of the dengue virus infectious vector DENV-2 through artificial inoculation. Materials and Methods: The research method used a quasi-experimental design with the intervention of injecting the DENV-2 virus intrathoracally. The study population was Ae. aegypti and Ae. albopictus laboratory colony females. The sample in the study was the 115th progeny in Ae. aegypti and the 45th progeny in Ae.albopictus. Results: Survival rate of Ae. aegypti with median survival 139.8 hours (day 5) and Ae. albopictus with median survival 87.7 hours (day 3): log rank p = 0.0001. The average gonotropic cycle of Ae. albopictus: 4 days and Ae aegypti 5 days, while the mean of metamorphosis in Ae. aegypti is 8 days and Ae. albopictus is 11 days. Conclusion: Ae. aegypti survive more than Ae. albopictus. Gonotropic cycle Ae. albopictus faster than Ae. aegypti while the metamorphosis time of Ae. aegypti was faster than that of Ae. albopictus. It is necessary to increase vector control through mosquito nest eradication activities, simultaneously for a maximum of 5 days, based on considerations of the survival rate and gonotropic cycle of infectious vectors.
