Comprehensive Genomic and Prognostic Analysis of the ADCY3 Genes in Gastric Cancer Genta Hafied Naga Samudra (1), Muhamad Salman Fareza (2), Sarmoko (3*)
1) Student, Department of Pharmacy, Faculty of Health Sciences, Jenderal Soedirman University, Indonesia
2) Lecturer, Department of Pharmacy, Faculty of Health Sciences, Jenderal Soedirman University, Indonesia
3) Lecturer, Department of Pharmacy, Faculty of Health Sciences, Jenderal Soedirman University. Dr. Soeparno street, Karangwangkal, Purwokerto, Central Java, Indonesia, 53123.
Email: sarmoko[at]unsoed.ac.id (corresponding author)
Abstract
Background: Adenylate cyclase, an essential enzyme in cell signaling. Adenylate cyclase comprises 12 isoforms, one of them is ADCY3. The previous study shows that ADCY3 expression was specifically altered in gastric cancer samples. However, the data of ADCY3 expression and prognosis on gastric cancer is still limited.
Purpose: To show prognosis and pathway of ADCY3 in gastric cancer by bioinformatics analysis.
Methods: The genetic mutations and expression were investigated using the Oncomine and cBioPortal databases. The prognostic value was assessed by the Kaplan-Meier plotter. Protein-protein interaction was investigated using STRING and Cytoscape. Gene Ontology and KEGG pathways were analyzed by using the Webgestalt database.
Results: The Oncomine analysis indicated that the expression level of ADCY3 in gastric adenocarcinoma was higher compared with normal tissue. By cBioportal analysis showed that gene amplification of ADCY3 occurred. High expression of ADCY3 correlated to worse overall survival in gastric cancer patients. Further analysis using KEEG and GO showed that ADCY3 involved in the relaxin pathway, apelin pathway, and chemokine pathway implicated in gastric cancer development.
Conclusion: In summary, high expression of ADCY3 correlated to worse overall survival in gastric cancer patients. The relaxin pathway, apelin pathway, and chemokine pathway implicated in gastric cancer development.
