PHYTHOCHEMICAL ANALISIS OF BRUGUIERA GYMNORHIZA STEM BARK AS ANTIOXIDANT AND APHA-GLYCOSIDASE INHIBITORS Warsinah(a*), Hartiwi Diasturi(b)
Faculty of Health and Sciences
Jenderal Soedirman University
Abstract
Gymnorhizza bruguiera is a mangrove plant used for traditional medicine. This plant contains alkaloid compounds, plavanoids, saponins, tannins and steroids. It has potential activity as a source of antioxidants and 945;-glucosidase inhibitors. Antioxidants are substrate oxidation inhibitors that are easily oxidized, whereas 945;-glucosidase inhibitors can limit the action of 945;-glucosidase that digest carbohydrates in the intestine. This study aims to determine the class of active compounds that function as antioxidants and 945;-glucosidase enzymes in vitro in Bruguiera gymnorhiza stem bark extract. This research includes extraction using n-hexane, ethyl acetate and methanol solvent, phytochemical tests with TLC method, antioxidant activity test with DPPH method, 945;-glycosidase inhibitor activity and active extracts are fractionated with preparative TLC, then active fractions are identified by GCMS.The results showed that the yield of n-hexane extract was 0.90%, ethyl acetate extract was 4.27% and methanol bark extract was 12.65%. Ethyl acetate and methanol extracts are a group of compounds that provide very strong antioxidant activity while n-Hexane extract is very weak. Ethyl acetate and methanol extracts provide inhibitory activity of 66.03% and 68.80%, at a concentration of 250 ppm while at glucobay 5 at 90.89%. Thin layer chromatography analysis produced the best eluent, methanol: DCM: n-hexane (2: 3: 1) and showed 5 fractions. Fraction 1 has 945;-glucosidase inhibiting activity with an IC50 value of 167.5 ppm. In fraction 1 hexadecanoic acid and phenol, 2,2 methylenebis were identified. Ethanol extract has the highest antioxidant activity and 945;-glucosidase inhibitors and the extract contains hexadecanoic acid and phenol, 2.2 methylenebis.
Keywords: B gymnorhiza; active compounds; inhibitors; antioxidants; α-glucosidase. Mangrove; stem bark
