IDENTIFICATION OF MICROORGANISMS ON SURGICAL INSTRUMENTS AFTER AUTOCLAVE STERILIZATION Achmad Samsudin, Thatit Nurmawati*
STIKes Patria Husada Blitar,
jl. S.Suriyadi 168 Blitar
*thatitnurmawati4[at]gmail.com
Abstract
Surgical wound infection is a complication of hospital treatment that is often found. Hospital instruments, operating room staff, operating room environment that is less sterile can be the cause of infection of surgical wounds. Efforts to prevent nosocomial infections using the autoclave sterilization method must be carried out. The purpose of this study was to identify instrument microorganisms after autoclave sterilization in Kanjuruhan Hospital District Malang. This type of research is quantitative with a descriptive approach to identifying microorganisms in basic surgical tool sets. The population in this study is the surgical instrument that is often used. The inclusion criteria for inclusion of tools have not been used for surgery and have been sterilized. The exclusion criteria are tools that have been dropped or touched by researchers or other things. Data collection by taking a sample (swab). Microorganism identification using Vitex. The results showed that the results of observation of the instrument after sterilization appeared on the IV day colony. Gram painting shows gram negative. the number of shears and tweezers colonies was 91% while the clamps were 87%. It is suspected that the stem bacterium is Sphingomonas paucimobilis. It is recommended to use the instrument no more than 3 days after sterilization.
