Antioxidant test of ethanolic extract of Chromolaena odorata leaves in vitro and in vivo Indah Solihah1, Inayatul Munawwaroh1, Herlina1, Riana Sari Puspita Rasyid2
1 Departement Of Pharmacy,Faculty of Mathematics and Natural Sciences,Sriwijaya University
2 Departement of Medicine, Faculty of Medicine, Sriwijaya University, Ogan Ilir 30662, South Sumatera, Indonesia
Abstract
Chromolaena odorata is a member of Asteraceae family contains flavonoids, tannins, saponins, and steroids. Flavonoid compounds are known to have antioxidant activity. This study aims to observe the antioxidant activity of the ethanolic extract of Chromolaena odorata leaves in vitro and in vivo. In vitro antioxidant activity used the DPPH test method. Determination of IC50 values in vitro test was carried out on extracts with concentrations of 50, 100, 200, 400, and 800 ppm. Whereas in vivo antioxidant activity used the TBARS test method. Tests carried out by measured levels of malondialdehyde (MDA) in male wistar strain induced paracetamol 2g/KgBW. Determination of ED50 in vivo test was carried out on extracts with doses of 125, 250, and 500 mg/KgBW. Vitamin C with dose 6,5mg/KgBW used as positive control and 1% of Na CMC used as negative control. IC50 value of ethanolic extract of Chromolaena odorata leaves against DPPH radical was 84.319ppm (strong antioxidant). In vivo test results showed a decrease in the levels of malondialdehyde in the positive control, negative control, and treatment group of ethanolic extract of Chromolaena odorata leaves with doses of 125, 250 and 500 mg/KgBW was 68.146%- 5.426%- 39.701%- 48.223% and 58.974% respectively. The decrease in MDA levels of rats that treated with ethanolic extract of Chromolaena odorata leaves was significantly different from the negative controls (p<0.05). Based on these results it can be concluded that the ethanolic extract of Chromolaena odorata leaves has antioxidant activity with ED50 was 312.241 mg/KgBW.
