Lowering Endogenous Nitric Oxide In Circulating Mature Progenitor Cells as The Stress Effect of Tobacco Smoking Habbit Titin Andri Wihastuti(a*), Wiwit Nurwidyaningtyas (b), Fibe Yulinda Cesa (c), Cholid Tri Tjahyono(d), Teuku Heriansyah(e)
a) Department of Biomedical Nursing Science, Faculty of Medicine, Brawijaya University, Malang, Indonesia
*titinwihastuti[at]gmail.com
b) Department of Biomedical Nursing Science, STIKES Kendedes, Malang, East Java, Indonesia
c) Master Program in Biomedical Science, Faculty of Medicine, Brawijaya University, Malang,East Java, Indonesia
d) Department of Cardiology, Faculty of Medicine, Brawijaya University, Malang, East Java, Indonesia;
e) Department of Cardiology and Vascular Medicine, School of Medicine, Syiah Kuala University/Zainoel Abidin General Hospital, Banda Aceh, 23111, Indonesia
Abstract
Background and objective. Nitrix oxide (NO) has important role in maintain cellular viability which can be altered by long term exposure of oxidative stress. NO reduction referred to effect the number of circulating endothelial progenitor cells (EPCs). Nicotine, highly toxic compound of tobacco exposure induced marked cell death, but there are still limited data regarding the effect to endogenous NO in circulating mature progenitor cells. The present study investigated the in vivo effect of tobacco smoking to the NO level in mature EPCs.
Methods. Pheriperal Blood Mono Nuclear Cells (PBMNCs) were isolated from 5 mL blood with Ficoll-based gradient centrifugation. endogenous NO was measure with NO-FCM orange dye positive cells in double marked CD41 and Cd62E. Targeted cells were enriched by the technique of fluorescense cell separation BD FACS Melody Cell Sorter. This study was support by 30 participants with history of 5 years of smoking more than 10 cigarettes per day.
Result.Our data demonstrated that mean NO level in the tobacco smoker is 9.7percent (\pm 1.9percent SD) lower than NO level in non-smoker participant in range 12.3percent (\pm 0.7percent SD). The resulting negative NO stained cell population contained 90.3percent (\pm 3.7percent SD) higher in the tobacco smoker.
Conclution. Our result show that tobacco smoking has effect to endogenous NO in mature EPCs swiftness in response to high nicotine exposure, may influence proportion of viable mature EPC in circulation.
