Analysis the Role of ARHGAP35 in Breast Cancer by the Bioinformatics Approach Dicky Rizky Febrian (a), Nur Amalia Choironi (a), Sarmoko (a*)
(a) Department of Pharmacy, Faculty of Health Sciences, Jenderal Soedirman University
Dr. Soeparno street, Karangwangkal, Purwokerto, Central Java, Indonesia, 53123.
*sarmoko[at]unsoed.ac.id
Abstract
Breast cancer is the second most common cause of cancer-related deaths worldwide after lung cancer. Rho GTP-ase activating protein 35 (ARHGAP35) is known to be implicated in breast cancer growth and migration. This study aims to analyze the role of ARHGAP35 in breast cancer by the bioinformatics approach. Oncomine was used to analyze gene expression, GEPIA was used to analyze overall survival, STRING and WEBGESTALT were used to analyze protein-protein interaction, gene ontology and KEGG were used to analyze the biological pathway. The expression of ARHGAP35 in several types of breast cancer showed higher expressions than in normal cells. The patients with high expression of ARHGAP in invasive breast carcinoma have lower overall survival. We found that ARHGAP35 interacted with RHOA, RHOB, RHOC, RHOD, RAC1, RND1, RASA1, FYN, SRC, and CDC42. Furthermore, ten protein was analyzed and they involved in some biological process such as maintenance of cytoskeleton polarity, regulation of cell shape, actin fillament process, and regulation of cell morphogenesis via adherens junctions, axon guidances, platelet activation, focal adhesion, regulation of actin cytoskeleton, tight junction, and sphingolipid signaling pathway. Taken together, ARHGAP35 has many roles in growth and migration of breast cancer with several pathways.
Keywords: ARHGAP35, breast cancer, overall survival, gene ontology, KEGG pathway
