Anti-inflammatory Effect of Zingiberaceae Herbals in Rats
Heny Ekowati*, Fitria Nur Malita Sari, Bellia Hasyim, Esti Dyah Utami, Eka Prasasti Nur Rachmani, Laksmi Maharani, Ika Mustikaningtias

Pharmacy Department, Faculty of Health Sciences, Universitas Jenderal Soedirman,
Jl. Dr. Suparno, Karangwangkal, Purwokerto 53123, Indonesia

Abstract

Zingiberaceae family, have potential effect as anti-inflammatory agent. The purpose of this study was to determine the anti-inflammatory effects of C. longa (CL), A. galanga (AG), Z. officinale (ZO), Z. cassumunar (ZC), and their combination extract in rats. The hit-paw udema method was used to examine the anti-inflammatory effect of the herbs. An experimental study design was set up with post only control group design. Rats were divided into five groups, 5-7 rats in each group. Group I, the solvent control group was given Tween orally- group II is the positive control group was given Na-diclofenac 4.5 mg/kgBW. The other three groups were given CL, AG, ZO, ZC and its combination, at the dosage of 500 mg/kgBW for single extract and 250 mg/kgBW each for combination extract. CL and AG extract was dissolved in Tween 1% and administered orally 1 h before carrageenan injection (sub-plantar 1 mL in rat foot). Observations of anti-inflammatory effects was performed using udema volume (mL), the percentage of increasing volume, Area Under Curve (AUC), anti-inflammatory effect percentage (% DAI) and motility score. Data were analyzed using one-way ANOVA followed by LSD. The percentage of DAI showed that the anti-inflammatory activity in combination extract was significantly higher than single extract. In CL and AG extract : 60,03 %- 50,11 %- 51,09 %, respectively. In ZO and ZC : 36%, 31% and 20%, respectively. Motility score data showed that extract combination has higher anti-inflammatory effect than their single extract. The combination and their single extract, CL and AG:1,74- 1,55- 1,52- respectively- in ZO and ZC : 1.29- 1.29- 1,26, respectively. This study indicated that combination of Curcuma longa and Alpinia galanga- Zingiber officinale and Zingiber cassumunar, can be developed as anti-inflammatory agent.

Keywords: Zingiberaceae, hit-paw udema method, rats

Topic: Pharmacy