Pregnancy Increases The Expression of Prostaglandin-2 in The Sacroiliac Joint (Study in-vivo Rattus Norvegicus Strain Wistar)
Author: Ika Oktaviani 1,2- Bambang Rahardjo 3,4, Endang Sri Wahyuni 6, Maya Devi Arifiandi 5,6, Titin Andri Wihastuti 7*

1. Student, Doctoral program of Medical Science, Faculty of Medicine, University of Brawijaya, Malang, Indonesia
2. Senior Lecture, Midwifery Study Program, Tanjungkarang Ministry of Health Polytechnic
3. Senior Lecture, Faculty of Medicine, University of Brawijaya, Malang, Indonesia
4. Assistant Professor, Department Obstetrics and Gynaecology, Syaiful Anwar Hospital, Malang, Indonesia
5. Assistant Professor, Department Obstetrics and Gynaecology, University Brawijaya Hospital, Malang, Indonesia
6. Lecture, Department of Midwifery, Faculty of Medicine, University of Brawijaya, Malang, Indonesia
7. Professor, Department of Nursing, Faculty of Medicine, University of Brawijaya, Malang, Indonesia
*Corresponding author :
email titinwihastuti[at]gmail.com,
official address: Medical Faculty University of Brawijaya Jalan Veteran Malang Indonesia

Abstract

Abstract
Background: Increased uterus weight during pregnancy moves down of gravity forward, increasing lumbar lordosis, excessive mechanical emphasis on pelvic, which is frequently causing pain. 1 in 5 pregnant women (20-30%) felt pain around the sacroiliac joint (SIJ), affecting the gait, and even being severe disability 8%. Mechanical emphasis on SIJ triggered local injury and PGE-2 are thought to be produced. PGE-2 enriched in injured tissue directly sensitizes nociceptors to cause central and peripheral sensitization, it also potentiates sensitizing effects of other pain mediators..

Purpose: This study aims to prove an increasing expression of PGE-2 in SIJ on pregnancy.

Methods: The research used a post-test only with a control group design. Animal experiments were 12 rats strain-wistar. The first stage of this study was the impregnating process through synchronization of the estrous phase. The next step was taking SIJ cartilage on day 19 of gestational age for the pregnant rat^s group and homogeneous age for the non-pregnant group. Analyze of data used independent t-test.

Results: the study found there was significant difference in the expression of PGE-2 on SIJ between pregnant and nonpregnant rats p-value 0,018 (< 0,05). Mean Indeks Remmele Score pregnants rats 6,16 0,833 (mean standard error of mean) were higher than nonpregnant rats 3,16 1,602 (CI 95% 0,640-5,360). Trauma to fibrous tissue and cartilage triggers the activation of silent nociceptors in the joints, and pro-inflammatory cytokines. Each of these can stimulate increased prostaglandins in SIJ.

Conclusion: pregnancy can increases PGE-2 expression in the fibrous tissue and cartilage of the sacroiliac joints

Keywords: PGE-2, pregnancy, rats, SIJ

Topic: Biomedical Sciences