Real Time Polymerase Chain Reaction Assay for Rapid Detection of Porcine Gelatin DNA in Soft Candy
Agustin Krisna Wardani and Reza

Department of Food Science & Technology, Faculty of Agricultural Technology, Universitas Brawijaya Malang

Abstract

Gelatin has been used in many food products including soft candies. Gelatin can be made from pork, beef or other animal. In 2017, imported candies in Indonesia reach 10.7 US Dollar. Reliable detection method of porcine gelatin DNA in soft candy is required for health and religious concerns especially in Indonesia, where the majority of the people are moslem. In this study, porcine-specific short amplicon-size for Real-Time Quantitative Polymerase Chain Reaction (qPCR) assay was used for the detection of porcine gelatin DNA in commercial imported soft candies. A pair of porcine-specific primers defining a 145 bp region of the mitochondrial D-loop 443 were used. Ten samples of imported soft candies were purchased from local store in Malang, Indonesia. The result showed that three samples were found to contain porcine gelatin DNA and the rest were found to be negative. We demonstrated that Real-Time Quantitative PCR (qPCR) method can be proposed as an effective method for the identification of porcine DNA in soft candies and other processed foods to support halal authentication in Indonesia.

Keywords: D-loop 443 primer, Halal authentication, Imported soft candies, Porcine DNA, Real-Time PCR

Topic: FOOD SAFETY AND SECURITY