The Potential of Robusta and Arabica Coffee Leaves Tea Kombucha as Hyperglycemia Therapeutic Agent in Streptozotocin-induced Diabetic Rats
Septi Darlia Putri*, Elok Zubaidah, Yunianta

Departemen of Food Science and Technology, Faculty of Agricultural Technology, Brawijaya University, Malang 65145, Indonesia
*septidarlia[at]gmail.com

Abstract

Kombucha is a drink made from stepping tea and sugar fermented by symbiotic bacteria and yeast (SCOBY). Commonly, kombucha is made from tea leaf (Camellia sinesis). The previous study showed that kombucha can be made from other plants containing high phenolics compounds, such as coffee leaf. This research used the old coffee leaves cultivar from Dampit, Malang Regency (Arabica and Robusta) as the raw material to produce kombucha. The study conducted to explore the potency of coffee leaf kombucha as hyperglycemia therapeutic agent in streptozotocin induced diabetic rats compared with green tea and black tea kombucha. Streptozotocin (STZ) induced diabetic rats were orally supplied with kombucha at dose of 5 ml/Kg body weight per day for 28 day. Fasting plasma glucose (FPG) was measured every week in day 0, 7, 14, 21 and 28. At the end of treatment, rats were sacrificed. Their bloods were collected and submitted to various biochemical measurement including superoxide dismutase (SOD) activity, malondialdehyde (MDA), total cholesterol (TC), high density lipoprotein (HDL) and low density lipoprotein (LDL) level. Their pancreas tissue was dissected to perform immunohistochemical (IHC) staining. The data obtained analyzed by analysis of variance (ANOVA) with a confidence interval of 95% and followed by BNJ test with a confidence interval of 95%. The results demonstrated that all kombucha (black tea, green tea, Arabica and Robusta coffee leaf tea) were capable of reducing FPG, MDA, TC LDL, TG, and as well as increasing HDL of STZ-induced diabetic Wistar rats. Nevertheless, the ability of black tea and Arabica coffee leaf tea kombucha to manage hyperglycemia and dyslipidemia appeared to be superior to others. All kombucha were also capable of regenerating pancreatic cells. Black tea kombucha provide the highest number of pancreatic beta cells, and significantly different from green tea and robusta coffee leaf tea kombucha. The finding revealed that kombucha administration induced attractive curative effects in diabetic rats and that can be considered as potential candidate for future application as functional food for treatment of diabetes.

Keywords: Coffee Leaf; Robusta; Arabica; Kombucha; Diabetes Mellitus; Hyperglycemia

Topic: HEALTH, NUTRITION AND MEDICAL MICROBIOLOGY