Motility, viable percentage and Abnormality of the sperms of frozen bali bull semen diluted by andromed and yolk tris N Salma1, A L Toleng2*, M Yusuf2, Masturi2, Sahiruddin2, U Masir3
1) Departement of Production, Faculty of Animal Science, Hasanuddin University, Makassar, Indonesia
2) Laboratory of Animal Reproduction Departement of Animal Production, Faculty of Animal Science, Hasanuddin University, Makassar, Indonesia
3) Study Program of livestock Agribissness, Departement of Animal Science, Politeknik Pertanian Negeri Pangkajene Kepulauan, jl. Poros Makassar pare-pare km. 83, Kec. Mandalle, Kab. Pangkep, Indonesia
Abstract
The objective of the research was to compare the quality of frozen cement (motility, sperm live-cell and spermatozoa abnormality) of Bali cattle on andromed and Tris egg yolk (TKT) diluent. This research used one of the Balinese bull. Cement was collected into five times for two diluent treatments, which is, P1 = Adromed + Aquadest and P2 = TKT + glycerol. The parameters measured were motility, sperm live-cell and abnormalities of spermatozoa. Each parameter in each treatment was compared using the T test (t-test independent sample). The results of this research indicate that P1 is better able to maintain with P2, the motility and the percentage of sperm live-cell frozen cement spermatozoa in Andromed diluent is still above 40%, is 50.8 , 6.14% and TKT is only 25.8 , 4.43%. For percentage of sperm live-cell, andromed had a value of 54.2 , 5.03% and a TKT of 33.3 , 2.64%. There was no a significant difference in the abnormalities of spermatozoa in frozen semen from Bali cows in the two diluents which the respective values for Andromed and TKT were 13.2 , 2.73% and 14.3 , 3.51%. It can be concluded that TKT still needs improvement to reach 40% or more.
Keywords: Abnormality, andromed, Motility, Viable Percentage, Yolk tris