Cytochrome B (Cyt B) mtDNA genetic diversity of the second filial (F2) of Banteng Bali cross Lellah Rahim1, Sri Rachma Aprilita Bugiwati1, Andi Ervin Adhianto1, Muhammad Ihsan Andi Dagong1, Nurlina Saking2, Set Pasino2 and Mayrawaty Arifin2
1Faculty of Animal Science, Hasanuddin University, Jl. Perintis Kemerdekaan KM 10 Makassar 90245, Indonesia.
2 UPT-PTHPT, South Sulawesi Livestock Services, Pucak Village Maros District, South Sulawesi Province Indonesia
Abstract
Bali cattle are native to Indonesia and are the result of domestication from Banteng, (Bos-bibos Banteng). The Cytochrome B gene is widely used in systematic studies to solve differences at many taxonomic levels and has been considered as one of the genes used in phylogenetic action. Cytochrome B is also very useful for comparing species in the same genus or the same family. Genetic characterization tests were carried out to identify the characteristics of mtDNA specific identifiers of Balinese cattle with Banteng (F2). The edited nucleotide sequences are then aligned using the Clustal W program contained in the MEGA 6 software. Construction of the phylogeny tree is carried out using the MEGA 6 program based on p-distance values. Phylogeny tree construction uses bootstrapped UPGMA method with 1000 repetitions. Based on genetic distance analysis, it shows that Bali cattle and Banteng F2 with Javanicus Bos have a genetic distance that is relatively the same as the genetic distance value (0,000 - 0.001) but has a great genetic distance with the Taurus Boss, Bos Indicus and Bubalus Buballis with genetic distance values ( 0.050 - 0.094). This shows that in Cytochrome B DNA, Bali cattle and Banteng F2 have identical genetic distances. Based on the UPGMA tree phylogeny method, the results show that the kinship group 1 (Bali cattle 03 and 07, Banteng (F2) 01, 07, 08, and 09 with Bos Javanus) and group 2 (Bali cattle 02, 04, 08, 09 and Banteng (F2) 10) are in the same branch showing that the results are relatively similar and have a close kinship. It can be concluded that the characteristics of DNA in the cytochrome B of Bali cattle and Banteng F2 are relatively the same and both have a very close relationship.
Keywords: Cyt B, genetic diversity, Bali cattle, Banteng, genetic distance
Topic: Animal genetics, Breeding and livestock production
